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Three-dimensional cellular ultrastructure resolved by X-ray microscopy

  1. Author:
    Schneider, G.
    Guttmann, P.
    Heim, S.
    Rehbein, S.
    Mueller, F.
    Nagashima, K.
    Heymann, J. B.
    Muller, W. G.
    McNally, J. G.

  2. Author Address

    [Schneider, Gerd; Guttmann, Peter; Heim, Stefan; Rehbein, Stefan] Helmholtz Zentrum Berlin Mat & Energie GmbH, Berlin, Germany. [Mueller, Florian; Mueller, Waltraud G.; McNally, James G.] NCI, Lab Receptor Biol & Gene Express, NIH, Bethesda, MD 20892 USA. [Nagashima, Kunio] NCI, Electron Microscopy Lab, SAIC Frederick Inc, NIH, Frederick, MD 21701 USA. [Heymann, J. Bernard] NIAMSD, Struct Biol Lab, NIH, Bethesda, MD 20892 USA.;Schneider, G, Helmholtz Zentrum Berlin Mat & Energie GmbH, Wilhelm Conrad Rontgen Campus, Berlin, Germany.;gerd.schneider@helmholtz-berlin.de mcnallyj@exchange.nih.gov
    1. Year: 2010
    2. Date: Dec
  2. Journal: Nature Methods
    1. 7
    2. 12
    3. Pages: 985-U116
  4. Type of Article: Article
  5. ISSN: 1548-7091
  1. Abstract:

    We developed an X-ray microscope using partially coherent object illumination instead of previously used quasi-incoherent illumination. The design permitted the incorporation of a cryogenic tilt stage, enabling tomography of frozen-hydrated, intact adherent cells. We obtained three-dimensional reconstructions of mouse adenocarcinoma cells at similar to 36-nm (Rayleigh) and similar to 70-nm (Fourier ring correlation) resolution, which allowed us to visualize the double nuclear membrane, nuclear pores, nuclear membrane channels, mitochondrial cristae and lysosomal inclusions.

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External Sources

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  2. DOI: 10.1038/nmeth.1533
  3. View record in Web of Science®
  4. WOS: 000284686300013

Library Notes

  1. Fiscal Year: FY2010-2011
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