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Cloning of guinea pig IL-4: Reduced IL-4 mRNA after vaccination or Mycobacterium tuberculosis infection

  1. Author:
    Jeevan, A.
    Yoshimura, T.
    Ly, L. H.
    Dirisala, V. R.
    McMurray, D. N.

  2. Author Address

    [Jeevan, Amminikutty; Ly, Lan H.; Dirisala, Vijaya R.; McMurray, David N.] Texas A&M Hlth Sci Ctr, Dept Microbial & Mol Pathogenesis, College Stn, TX 77843 USA. [Yoshimura, Teizo] NCI, Mol Immunoregulat Lab, Canc & Inflammat Program, Ctr Canc Res, Frederick, MD 21702 USA.;Jeevan, A, Texas A&M Hlth Sci Ctr, Dept Microbial & Mol Pathogenesis, 407 Reynolds Med Bldg, College Stn, TX 77843 USA.;ajeevan@medicine.tamhsc.edu
    1. Year: 2011
    2. Date: Jan
  2. Journal: Tuberculosis
    1. 91
    2. 1
    3. Pages: 47-56
  4. Type of Article: Article
  5. ISSN: 1472-9792
  1. Abstract:

    Interleukin-4 (IL-4), a pleiotropic cytokine produced by T-helper type 2 (Th2) cells, is involved in promoting humoral immune responses, allergic reactions and asthma. Previous studies suggested an important role for IL-4 in susceptibility to pulmonary tuberculosis; however, the role of IL-4 has not been studied in the guinea pig, a highly relevant model for this disease. In the present study, we cloned a cDNA for guinea pig IL-4 and examined, for the first time, mRNA expression by real-time RT-PCR in cultured guinea pig cells. High levels of IL-4 mRNA expression were detected in spleen T cells of naive animals after in vitro stimulation with PMA plus ionomycin for 4-24 h. The expression of IL-4 mRNA was low in spleen and lymph node cells immunized with ovalbumin (OVA) plus Complete Freund's Adjuvant (CFA) in response to OVA (Th1), but significantly higher in the guinea pigs immunized with OVA plus alum (Th2). BCG vaccination reduced the expression of IL-4 mRNA in both spleen and lung digest cells compared to naive guinea pigs, while levels of IFN-gamma were similar in both groups. Furthermore, lung cells from Mycobacterium tuberculosis-infected guinea pigs stimulated in vitro with PPD or MPT64 showed low levels of IL-4 mRNA expression. Thus, BCG vaccination or M. tuberculosis infection modulates IL-4 mRNA expression in the guinea pig. Cloning of guinea pig IL-4 will allow us to address the role of IL-4 in vaccine-induced resistance to pulmonary TB in a highly relevant animal model. (C) 2010 Elsevier Ltd. All rights reserved.

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External Sources

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  2. DOI: 10.1016/j.tube.2010.11.006
  3. View record in Web of Science®
  4. WOS: 000286876700007

Library Notes

  1. Fiscal Year: FY2010-2011
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