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An allelic discrimination SNP assay for distinguishing the mitochondrial lineages of European wildcats and domestic cats

  1. Author:
    McEwing, R.
    Kitchener, A. C.
    Holleley, C.
    Kilshaw, K.
    O'Donoghue, P.

  2. Author Address

    [McEwing, Ross] Royal Zool Soc Scotland, WildGenes Lab, Edinburgh EH12 6TS, Midlothian, Scotland. [Kitchener, Andrew C.] Natl Museum Scotland, Dept Nat Sci, Edinburgh EH1 1JF, Midlothian, Scotland. [Holleley, Clare] NCI, Lab Genom Div, Frederick, MD 21702 USA. [Kilshaw, Kerry] Univ Oxford, Wildlife Conservat Res Unit, Dept Zool, Oxford OX13 5QL, Oxon, England. [O'Donoghue, Paul] Univ Chester, Dept Biol Sci, Chester CH1 4BJ, Cheshire, England.;McEwing, R (reprint author), Royal Zool Soc Scotland, WildGenes Lab, 134 Corstorphine Rd, Edinburgh EH12 6TS, Midlothian, Scotland;rmcewing@rzss.org.uk
    1. Year: 2012
    2. Date: Mar
  2. Journal: Conservation Genetics Resources
    1. 4
    2. 1
    3. Pages: 163-165
  4. Type of Article: Article
  5. ISSN: 1877-7252
  1. Abstract:

    Here we present an allelic discrimination assay designed to distinguish European wildcat mtDNA lineages from those of the domestic cat. Introgression between the native wildcat and introduced domestic cat has the potential to limit and reduce the recent recovery of remnant populations. Applied conservation genetic techniques can aid current conservation decisions on lethal control and neutering measures in hybrid zones. This real-time PCR technique offers a rapid, inexpensive and reliable assay to assess mtDNA introgression in the wild and has already identified hybrid individuals in the Scottish wildcat captive breeding programme.

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External Sources

  1. View Full Text
  2. DOI: 10.1007/s12686-011-9499-z
  3. View record in Web of Science®
  4. WOS: 000300772500039

Library Notes

  1. Fiscal Year: FY2011-2012
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