Skip NavigationSkip to Content
Return Return to Search Results

Collection and preparation of rodent embryonic samples for transcriptome study

  1. Author:
    Golubeva, Y.
    Symer, D.

  2. Author Address

    Histotechnology Laboratory, SAIC, Frederick National Lab of Cancer Research, NCI, NIH, Frederick, MD, USA.
    1. Year: 2014
    2. Epub Date: 12/10/2013
  2. Journal: Methods in molecular biology (Clifton, N.J.)
    1. 1092
    2. Pages: 297-356
  4. Type of Article: Article
  5. ISSN: 1940-6029 (Electronic) 1064-3745 (Linking)
  1. Abstract:

    The need for large-scale collection of rodent embryos and individual embryonic tissues for genomic and proteomic studies requires modification of traditional practices of embryo necropsy. The sample intended for transcriptome study should be rapidly dissected and stabilized to preserve its molecular integrity. The retrieval of high-quality RNA, DNA, and proteins from the target tissue is crucial for informative molecular analysis (e.g., gene profiling on microarray platform). We present a reliable method of collection and preparation of rodent embryos for genomic studies supported by detailed protocols and RNA extraction results for different stages of mouse embryonic development.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1007/978-1-60327-292-6_20
  3. PMID: 24318829

Library Notes

  1. Fiscal Year: FY2013-2014
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel