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Isolation and Functional Analysis of Human Neutrophils

  1. Author:
    Kuhns, D. B.
    Long Priel, D. A.
    Chu, J.
    Zarember, K. A.

  2. Author Address

    Neutrophil Monitoring Lab, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland. Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
    1. Year: 2015
    2. Date: 2-Nov
    3. Epub Date: 11/4/2015
  2. Journal: Current Protocols in Immunology
    1. 111
    2. Pages: 7.23.1-16
  4. Type of Article: Article
  5. ISSN: 1934-3671
  1. Abstract:

    This unit describes the isolation of human polymorphonuclear neutrophils (PMN) from blood using dextran sedimentation and Percoll or Ficoll-Paque density gradients. Assays of neutrophil functions including respiratory burst activation, phagocytosis, and microbial killing are also described.

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External Sources

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  2. DOI: 10.1002/0471142735.im0723s111
  3. PMID: 26528633
  4. PMCID: PMC4668800
  5. NIHMSID: NIHMS735782

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