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Regulation of mRNA export by nutritional status in fission yeast

  1. Author:
    Whalen, W. A.
    Yoon, J. H.
    Shen, R. L.
    Dhar, R.

  2. Author Address

    Dhar R NCI, Basic Sci Lab, NIH Bldg 41,Rm B506 Bethesda, MD 20892 USA NCI, Basic Sci Lab, NIH Bethesda, MD 20892 USA NCI, ABL Basic Res Program, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA
    1. Year: 1999
  2. Journal: Genetics
    1. 152
    2. 3
    3. Pages: 827-838
  4. Type of Article: Article
  1. Abstract:

    We have isolated a mutation in nup184(nup184-1) that is synthetically lethal with the mRNA export defective rae1-167 mutation in Schizosaccharomyces pombe. The consequence of the synthetic lethality is a defect in mRNA export. The predicted Nup184p is similar to Nup188p of Saccharomyces cerevisiae, and a Nup184-GFP fusion localizes to the nuclear periphery in a punctate pattern. The Delta nup184 null mutant is viable and also is synthetically lethal with rae1-167. In a rae1(+) background, both the nup184-1 and Delta nup184 mutations confer sensitivity to growth in nutrient-rich medium (YES) that is accompanied by nuclear poly(A)(+) RNA accumulation. Removal of the cAMP-dependent protein kinase, Pka1p, relieved the growth and mRNA export defects of nup184 mutants when grown in nutrient-rich medium. The activation of Pka1p is necessary, but not sufficient, to cause the severe poly(A)(+) RNA export defects when nup184 mutant cells are incubated in YES, suggesting nutritional status carl also regulate poly(A)(+) RNA export. Our results suggest that the regulation of poly(A)(+) RNA export by Pka1p kinase appears to be indirect, via a translation-dependent step, but post-translationally; in response to YES. [References: 53]

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