Skip NavigationSkip to Content

Persistent infection of rhesus macaques by the Rev-independent Nef(-) simian immunodeficiency virus SIVmac239: Replication kinetics and genomic stability

  1. Author:
    von Gegerfelt, A. S.
    Liska, V.
    Ray, N. B.
    McClure, H. M.
    Ruprecht, R. M.
    Felber, B. K.
  2. Author Address

    Felber BK NCI, ABL Basic Res Program, FCRDC, Human Retrovirus Pathogenesis Grp Bldg 535,Rm 110 Frederick, MD 21702 USA NCI, ABL Basic Res Program, FCRDC, Human Retrovirus Pathogenesis Grp Frederick, MD 21702 USA Dana Farber Canc Inst Boston, MA 02115 USA Harvard Univ, Sch Med, Dept Med Boston, MA 02115 USA Emory Univ, Yerkes Reg Primate Res Ctr Atlanta, GA 30322 USA
    1. Year: 1999
  1. Journal: Journal of Virology
    1. 73
    2. 7
    3. Pages: 6159-6165
  2. Type of Article: Article
  1. Abstract:

    We generated previously a Nef(-), replication-competent clone of SIVmac239 in which the Rev protein and the Rev-responsive element were replaced by the constitutive transport element (CTE) of simian retrovirus type 1 (A. S. von Gegerfelt and B. K. Feliber, Virology 232:291-299, 1997). In the present report, we show that this virus was able to infect and replicate in rhesus macaques. The Rev-independent Nef(-) simian immunodeficiency virus induced a persistent humoral immune response in all monkeys, although viral loads were very low. Upon propagation in the monkeys, the genotype remained stable and the virus retained its in vitro growth characteristics. The infected monkeys showed normal hematological values and no signs of disease at more than 18 months post-virus exposure. Therefore, replacement of the essential Rev regulation by the CTE generated a virus variant that retained its replicative capacity both in vitro and in vivo, albeit at low levels. [References: 30]

    See More

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel