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Identification of podocalyxin-like protein 1 as a novel cell surface marker for hemangioblasts in the murine aorta-gonad-mesonephros region

  1. Author:
    Hara, T.
    Nakano, Y.
    Tanaka, M.
    Tamura, K.
    Sekiguchi, T.
    Minehata, K.
    Copeland, N. G.
    Jenkins, N. A.
    Okabe, M.
    Kogo, H.
    Mukouyama, Y.
    Miyajima, A.

  2. Author Address

    Hara T Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku 1-1-1 Yayoi Tokyo 1130032 Japan Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku Tokyo 1130032 Japan Univ Tokyo, Core Res Evolut Sci & Technol, Bunkyo Ku Tokyo 1130032 Japan Tokyo Univ Pharm & Life Sci Hachioji Tokyo 1920355 Japan NCI, Mammalian Genet Lab, ABL Basic Res Program, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA Osaka Univ, Genome Informat Res Ctr Suita Osaka 5650871 Japan
    1. Year: 1999
  2. Journal: Immunity
    1. 11
    2. 5
    3. Pages: 567-578
  4. Type of Article: Article
  1. Abstract:

    Recent studies with avian embryos and murine embryonic stem cells have suggested that hematopoietic cells are derived from hemangioblasts, the common precursors of hematopoietic and endothelial cells. We molecularly cloned podocalyxin-like protein 1 (PCLP1) as a novel surface marker for endothelial-like cells in the aorta-gonad-mesonephros (AGM) region of mouse embryos, where long-term repopulating hematopoietic stem cells (LTR-HSCs) are known to arise. PCLP1(+) CD45(-) cells in the AGM region incorporated acetylated low-density lipoprotein and produced both hematopoietic and endothelial cells when cocultured with OP9 stromal cells. Moreover, multiple lineages of hematopoietic cells were generated in vivo when PCLP1(+)CD45(-) cells were injected into neonatal liver of busulfan-treated mice. Thus, PCLP1 can be used to separate hemangioblasts that give rise to LTR-HSCs. [References: 49]

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