Development and interlaboratory evaluation of a NIST Reference Material RM 8366 for EGFR and MET gene copy number measurements

Author:

He, Hua-Jun

Das,Biswajit

Cleveland, Megan H

Chen,Li

Camalier,Corinne

Liu, Liang-Chun

Norman, Kara L

Fellowes, Andrew P

McEvoy, Christopher R

Lund, Steve P

Almeida, Jamie

Steffen, Carolyn R

Karlovich,Chris

Williams,Mickey

Cole, Kenneth D
Author Address

Biosystems and Biomaterials Division, National Institute of Standards and Technology, 100 Bureau Drive, MS 8312, Gaithersburg, MD 20899, USA, Phone: +301-975-2169, Fax: +301-330-3447., Molecular Characterization and Clinical Assay Development Laboratory, Frederick National Laboratory for Cancer Research, Frederick, MD, USA., Biomolecular Measurement Division, National Institute of Standards and Technology, Gaithersburg, MD, USA., Thermo Fisher Scientific, Fremont, CA, USA., Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia., Statistical Engineering Division, National Institute of Standards and Technology, Gaithersburg, MD, USA., Biosystems and Biomaterials Division, National Institute of Standards and Technology, Gaithersburg, MD, USA.,

Abstract:

Background: The National Institute of Standards and Technology (NIST) Reference Material RM 8366 was developed to improve the quality of gene copy measurements of EGFR (epidermal growth factor receptor) and MET (protooncogene, receptor tyrosine kinase), important targets for cancer diagnostics and treatment. The reference material is composed of genomic DNA prepared from six human cancer cell lines with different levels of amplification of the target genes.

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