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Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

  1. Author:
    Cheng, Robert Y S
    Patel,Nimit
    Back, Timothy
    Basudhar,Debashree
    Somasundaram,Veena
    Kalen,Joseph
    Wink,David
    Ridnour,Lisa

  2. Author Address

    Molecular Mechanism Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute at Frederick., Small Animal Imaging Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc., Molecular Mechanism Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute at Frederick; ridnourl@mail.nih.gov.,
    1. Year: 2020
    2. Date: Mar 20
    3. Epub Date: 2020 03 20
  2. Journal: Journal of visualized experiments : JoVE
    1. 157
  4. Type of Article: Article
  5. Article Number: e60316
  6. ISSN: 1940-087X
  1. Abstract:

    Triple-negative breast cancer (TNBC) is an aggressive breast cancer subtype with limited therapeutic options. When compared to patients with less aggressive breast tumors, the 5-year survival rate of TNBC patients is 77% due to their characteristic drug-resistant phenotype and metastatic burden. Toward this end, murine models have been established aimed at identifying novel therapeutic strategies limiting TNBC tumor growth and metastatic spread. This work describes a practical guide for the TNBC orthotopic model where MDA-MB-231 breast cancer cells suspended in a basement membrane matrix are implanted in the fourth mammary fat pad, which closely mimics the cancer cell behavior in humans. Measurement of tumors by caliper, lung metastasis assessment via in vivo and ex vivo imaging, and molecular detection are discussed. This model provides an excellent platform to study therapeutic efficacy and is especially suitable for the study of the interaction between the primary tumor and distal metastatic sites.

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External Sources

  1. View Full Text
  2. DOI: 10.3791/60316
  3. PMID: 32250353
  4. View record in Web of Science®
  5. WOS: 000523286100017

Library Notes

  1. Fiscal Year: FY2019-2020
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