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Impact of Preanalytical Factors on the Measurement of Tumor Tissue Biomarkers Using Immunohistochemistry

  1. Author:
    Bagchi, Aditi
    Madaj, Zachary
    Engel, Kelly B
    Guan, Ping
    Rohrer, Daniel C
    Valley, Dana R
    Wolfrum, Emily
    Feenstra, Kristin
    Roche,Nancy
    Hostetter, Galen
    Moore, Helen M
    Jewell, Scott D

  2. Author Address

    Pathology and Biorepository Core, Van Andel Institute, Grand Rapids, Michigan., Spectrum Health Helen DeVos Children 39;s Hospital, Grand Rapids, Michigan., St. Jude Children 39;s Research Hospital, Memphis, Tennessee., Bioinformatics and Biostatistics Core, Van Andel Institute, Grand Rapids, Michigan., Preferred Solutions Group, Rockville, Maryland., Biorepositories and Biospecimen Research Branch, National Cancer Institute, Bethesda, Maryland., Pathology and Biorepository Core., Leidos Biomedical Research, Inc., Frederick, Maryland.,
    1. Year: 2021
    2. Date: May
    3. Epub Date: 2021 03 01
  2. Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
    1. 69
    2. 5
    3. Pages: 297-320
  4. Type of Article: Article
  5. Article Number: 22155421995600
  6. ISSN: 0022-1554
  1. Abstract:

    Analysis of formalin-fixed paraffin-embedded (FFPE) tissue by immunohistochemistry (IHC) is commonplace in clinical and research laboratories. However, reports suggest that IHC results can be compromised by biospecimen preanalytical factors. The National Cancer Institute 39;s Biospecimen Preanalytical Variables Program conducted a systematic study to examine the potential effects of delay to fixation (DTF) and time in fixative (TIF) on IHC using 24 cancer biomarkers. Differences in IHC staining, relative to controls with a DTF of 1 hr, were observed in FFPE kidney tumor specimens after a DTF of =2 hr. Reductions in H-score and/or staining intensity were observed for c-MET, p53, PAX2, PAX8, pAKT, and survivin, whereas increases were observed for RCC1, EGFR, and CD10. Prolonged TIF of 72 hr resulted in significantly reduced H-scores of CD44 and c-Met in kidney tumor specimens, compared with controls with 12-hr TIF. An elevated probability of altered staining intensity due to DTF was observed for nine antigens, whereas for prolonged TIF an elevated probability was observed for one antigen. Results reported here and elsewhere across tumor types and antigens support limiting DTF to =1 hr when possible and fixing tissues in formalin for 12-24 hr to avoid confounding effects of these preanalytical factors on IHC.

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External Sources

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  2. DOI: 10.1369/0022155421995600
  3. PMID: 33641490
  4. View record in Web of Science®
  5. WOS: 000645998900001

Library Notes

  1. Fiscal Year: FY2020-2021
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