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Cross-linking mass spectrometry for mapping protein complex topologies in situ

  1. Author:
    Lee,Kitaik [ORCID]
    O'Reilly,Francis [ORCID]

  2. Author Address

    Center for Structural Biology, Center for Cancer Research, National Cancer Institute (NCI), Frederick, MD 21702-1201, U.S.A.,
    1. Year: 2023
    2. Date: Feb 03
    3. Epub Date: 2023 02 03
  2. Journal: Essays in Biochemistry
  4. Type of Article: Article
  1. Abstract:

    Cross-linking mass spectrometry has become an established technology to provide structural information on the topology and dynamics of protein complexes. Readily accessible workflows can provide detailed data on simplified systems, such as purified complexes. However, using this technology to study the structure of protein complexes in situ, such as in organelles, cells, and even tissues, is still a technological frontier. The complexity of these systems remains a considerable challenge, but there have been dramatic improvements in sample handling, data acquisition, and data processing. Here, we summarise these developments and describe the paths towards comprehensive and comparative structural interactomes by cross-linking mass spectrometry. © 2023 The Author(s).

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External Sources

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  2. DOI: 10.1042/EBC20220168
  3. PMID: 36734207
  4. PII : 232526

Library Notes

  1. Fiscal Year: FY2022-2023
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