Posttranscriptional Regulation By Rev Protein of Human Immunodeficiency Virus Type 1 Results in Nonrandom Nuclear Localization of Gag Mrna

Author:

Romanov, V. I.

Zolotukhin, A. S.

Aleksandroff, N. N.

Dasilva, P. P.

Felber, B. K.
Author Address

Felber BK NCI FREDERICK CANC RES & DEV CTR HUMAN RETROVIRUS PATHOGENESIS GRP ABL BASIC RES PROGRAM FREDERICK, MD 21702 USA NCI FREDERICK CANC RES & DEV CTR HUMAN RETROVIRUS PATHOGENESIS GRP ABL BASIC RES PROGRAM FREDERICK, MD 21702 USA NCI FREDERICK CANC RES & DEV CTR MATH BIOL LAB MEMBRANE BIOL SECT FREDERICK, MD 21702 USA NCI FREDERICK CANC RES & DEV CTR MATH BIOL LAB MEMBRANE BIOL SECT FREDERICK, MD 21702 USA

Abstract:

The expression of the human immunodeficiency virus type I mRNAs containing the Rev-responsive element is regulated at the posttranscriptional level by the viral Rev protein. Rev increases the nucleocytoplasmic export of these mRNAs, leading to high expression. Using in situ hybridization and electron microscopy, we investigated the localization of a subgenomic gag mRNA in the absence and presence of Rev. In addition to the previously shown cytoplasmic accumulation of the Rev-dependent mRNA, we observed that in the presence of Rev the nuclear gag mRNA accumulates nonrandomly and forms specific localization patterns at the nuclear membrane and in the nucleoplasm. Cellular mRNAs for beta-actin and glyceralde-hyde-3-phosphate dehydrogenase were not found to form such patterns. These data suggest that Rev leads the gag mRNA to specific subnuclear locations, which further supports the transport function of Rev. (C) 1997 Academic Press. [References: 52]

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