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Isolation of single cells from individual mouse ovaries for flow cytometry and functional analysis

  1. Author:
    Bafor,Enitome
    Martin,Toni
    Carrell,Jeff
    Karwan,Megan
    Kimmel,Adrienne
    Omogiade, Uyi G
    Sanford,Michael
    Young,Howard
    Valencia,Julio

  2. Author Address

    Cancer Innovation Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA. Electronic address: enitome.bafor@nih.gov., Basic Science Program, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD, 21702, USA.,
    1. Year: 2023
    2. Date: Nov 13
    3. Epub Date: 2023 11 13
  2. Journal: STAR Protocols
    1. 4
    2. 4
    3. Pages: 102710
  4. Type of Article: Article
  5. Article Number: 102710
  1. Abstract:

    Here, we present a validated workflow to isolate sufficient viable single ovary cells from a single mouse without the need to pool from several mice. We provide steps essential for estrous staging, ovary harvesting and dissociation, ovary cell staining, data collection, and analysis. Our approach allows the use of these single-cell suspensions for flow sorting, flow cytometry analysis, or functional in vitro assays. Importantly, our protocol is designed to maximize the isolation of immune cells, including T cell subsets. Copyright © 2023. Published by Elsevier Inc.

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External Sources

  1. View Full Text
  2. DOI: 10.1016/j.xpro.2023.102710
  3. PMID: 37963022
  4. PII : S2666-1667(23)00677-9

Library Notes

  1. Fiscal Year: FY2023-2024
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