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Escherichia coli grown in inexpensive conical flat-bottom polypropylene tubes produce a high level of pUC vector

  1. Author:
    Marquez,Mayra
    Chen,Qian
    Cachaco,Silvia
    Yang,Jun
    Sui,Hongyan
    Imamichi,Tomozumi
  2. Author Address

    Laboratory of Human Retrovirology and Immunoinformatics, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA., Laboratory of Human Retrovirology and Immunoinformatics, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA. Electronic address: timamichi@mail.nih.gov.,
    1. Year: 2024
    2. Date: Jul 14
    3. Epub Date: 2024 07 12
  1. Journal: Journal of Microbiological Methods
    1. 224
    2. Pages: 106990
  2. Type of Article: Article
  3. Article Number: 106990
  1. Abstract:

    The pUC-derived plasmid yield from E. coli using polypropylene tubes (PP) was compared among round and conical tubes. The yield from cells grown in a cheaper conical-PP with flat-bottom was 1.5-fold higher (p < 0.001) than other PP. The use of the conical-PP can save research budgets in the current inflationary environment.

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External Sources

  1. DOI: 10.1016/j.mimet.2024.106990
  2. PMID: 39004285
  3. PII : S0167-7012(24)00102-7

Library Notes

  1. Fiscal Year: FY2023-2024
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