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Quantitative measurement of fusion of HIV-1 and SIV with cultured cells using photosensitized labeling

  1. Author:
    Raviv, Y.
    Viard, M.
    Bess, J.
    Blumenthal, R.

  2. Author Address

    NCI, Canc Res Ctr, Lab Expt & Computat Biol, POB B, Bldg 469, Room 216A, Miller Dr, Frederick, MD 21702 USA. NCI, Canc Res Ctr, Lab Expt & Computat Biol, Frederick, MD 21702 USA. Intramural Res Support Program, Ft Detrick, MD 21702 USA. SAIC, Aids Vaccine Program, Ft Detrick, MD 21702 USA. Blumenthal R NCI, Canc Res Ctr, Lab Expt & Computat Biol, POB B, Bldg 469, Room 216A, Miller Dr, Frederick, MD 21702 USA.
    1. Year: 2002
  2. Journal: Virology
    1. 293
    2. 2
    3. Pages: 243-251
  4. Type of Article: Article
  1. Abstract:

    The fusion of HIV and SIV with biological membranes was studied by photosensitized activation of a hydrophobic probe, [I- 125]iodonaphthylazide ([I-125]INA), by a fluorescent lipid which is situated in the target membrane. Photosensitized labeling of viral envelope-resident proteins occurs only upon their insertion into target membranes. Photosensitized labeling as a result of HIV-1 Env-mediated cell fusion showed the same kinetics as aqueous dye transfer. We have for the first time measured kinetics of HIV and SIV virus-cell fusion. HIV-1(MN) virions were about 10X less fusion active than SIVmne virions. SIV inactivated by aldrithiol-2 retained fusion activity similar to that seen with untreated virus. The relatively slow time course of SIV-cell fusion (t(1/2) = 19 min) indicates that the fusion events are stochastic. This feature provides a basis for understanding the mode of action of HIV/SIV entry inhibitors that target transition states. (C) 2002 Elsevier Science (USA).

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