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A sheathless nanoflow electrospray interface for on-line capillary electrophoresis mass spectrometry

  1. Author:
    Janini, G. M.
    Conrads, T. P.
    Wilkens, K. L.
    Issaq, H. J.
    Veenstra, T. D.

  2. Author Address

    NCI, Analyt Chem Lab, SAIC Frederick Inc, Frederick, MD 21702 USA NCI, Analyt Chem Lab, SAIC Frederick Inc, Frederick, MD 21702 USA Janini GM NCI, Analyt Chem Lab, SAIC Frederick Inc, Frederick, MD 21702 USA
    1. Year: 2003
  2. Journal: Analytical Chemistry
    1. 75
    2. 7
    3. Pages: 1615-1619
  4. Type of Article: Article
  1. Abstract:

    A novel, rugged capillary electrophoresis-electrospray ionization (CE-ESI) interface where the separation column, an electrical porous junction, and the spray tip are integrated on a single piece of a fused-silica capillary is described. ESI is accomplished by applying an electrical potential through an easily prepared porous junction across a 3-4-mm length of fused silica. A stable electrospray is produced at nanoflow rates generated in the capillary by electrophoretic and electroosmotic forces. The interface is particularly well suited for the detection of low-femtomole levels of proteins and peptides. The ruggedness of this interface was evident by the continuous operation of the same column for over a 2-week period with no detectable deterioration in separation or electrospray performance. The new interface was used for the LC-ESI-MS separation and analysis of peptides and proteins. Injection of 25 fmol of [Glu(1)]-fibrinopeptide B using the new device produced a CE-ESI-MS electropherogram with a signal-to- noise ratio of over 100 for this peptide.

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