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Effects of tRNA(3)(Lys) aminoacylation on the initiation of HIV-1 reverse transcription

  1. Author:
    Rigourd, M.
    Bec, G.
    Benas, P.
    Le Grice, S. F. J.
    Ehresmann, B.
    Ehresmann, C.
    Marquet, R.

  2. Author Address

    Univ Strasbourg 1, CNRS, Unite Propre Rech 9002, IBMC, 15 Rue Rene Descartes, F-67084 Strasbourg, France Univ Strasbourg 1, CNRS, Unite Propre Rech 9002, IBMC, F-67084 Strasbourg, France NCI, HIV Drug Resistance Program, Resistance Mech Lab, Frederick, MD 21702 USA Marquet R Univ Strasbourg 1, CNRS, Unite Propre Rech 9002, IBMC, 15 Rue Rene Descartes, F-67084 Strasbourg, France
    1. Year: 2003
  2. Journal: Biochimie
    1. 85
    2. 5
    3. Pages: 521-525
  4. Type of Article: Article
  1. Abstract:

    HIV-1 utilizes cellular tRNA(3)(Lys) to prime the initiation of reverse transcription. The selective incorporation of cytoplasmic tRNA(3)(Lys) into HIV-1 particles was recently shown to involve the lysyl-tRNA synthetase, and hence, the encapsidated tRNA(3)(Lys) is likely to be aminoacylated. Here, we tested the effect of aminoacylation on the initiation of reverse transcription. We show that HIV-1 reverse transcriptase is unable to extend lysyl-tRNA(3)(Lys). In addition, the viral polymerase does not significantly enhance the rate of tRNA deacylation, in contrast with previous studies on avian retroviruses. Thus, aminoacylation of the primer tRNA might prevent the initiation of HIV-1 reverse transcription from taking place before viral budding and maturation. (C) 2003 Editions scientifiques et medicales Elsevier SAS and Societe francaise de biochimie et biologic moleculaire. All rights reserved.

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