Skip NavigationSkip to Content
Return Return to Search Results

Characterization of an interleukin-7-dependent thymic cell line derived from a p53(-/-) mouse

  1. Author:
    Kim, K.
    Khaled, A. R.
    Reynolds, D.
    Young, H. A.
    Lee, C. K.
    Durum, S. K.

  2. Author Address

    NCI, Mol Immunoregulat Lab, Ctr Canc Res, Bldg 560,Rm 31-71, Ft Detrick, MD 21702 USA NCI, Mol Immunoregulat Lab, Ctr Canc Res, Ft Detrick, MD 21702 USA NCI, Expt Immunol Lab, Ctr Canc Res, Ft Detrick, MD 21702 USA Durum SK NCI, Mol Immunoregulat Lab, Ctr Canc Res, Bldg 560,Rm 31-71, Ft Detrick, MD 21702 USA
    1. Year: 2003
  2. Journal: Journal of Immunological Methods
    1. 274
    2. 1-2
    3. Pages: 177-184
  4. Type of Article: Article
  1. Abstract:

    In order to study the response of T cells to IL-7, we aimed to generate an IL-7-dependent thymocyte line. CD4(-)CD8(- )thymocytes from a p53(-)/(-) mouse were continuously propagated in interleukin-7 (IL-7), and after 2 months there developed an immortal line termed "D1." The D1 line has retained a stable dependency on IL-7. Withdrawal of IL-7 from D1 cells induced arrest in G1 phase of the cell cycle, followed by apoptosis. In addition to IL-7, several other cyrokines that employ gamma(e) as part of their receptor were also capable of stimulating D I cell survival and proliferation. Gene induction by IL-7 was analyzed in D1 cells using RNase protection and array analysis and revealed a number of transcripts potentially involved in cell cycle, apoptosis and signaling. (C) 2002 Elsevier Science B.V. All rights reserved.

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel