Skip NavigationSkip to Content
Return Return to Search Results

Binding characteristics of IFN-alpha subvariants to IFNAR2-EC and influence of the 6-histidine tag

  1. Author:
    Schmeisser, H.
    Kontsek, P.
    Esposito, D.
    Gillette, W.
    Schreiber, G.
    Zoon, K. C.

  2. Author Address

    NIAID, NIH, Bethesda, MD 20892 USA. Inst Neuroimmunol, SAS, Bratislava, Slovakia. NCI, SAIC, Frederick, MD 21702 USA. Weizmann Inst Sci, IL-76100 Rehovot, Israel.;Schmeisser, H, NIAID, NIH, 50 Ctr Dr, Bethesda, MD 20892 USA.;hschmeisser@niaid.nih.gov
    1. Year: 2006
    2. Date: Dec
  2. Journal: Journal of Interferon and Cytokine Research
    1. 26
    2. 12
    3. Pages: 866-876
  4. Type of Article: Article
  5. ISSN: 1079-9907
  1. Abstract:

    The expression, purification, detection, and assay of recombinant proteins have been made more convenient and rapid by the use of small affinity tags. To facilitate the purification of interferon-alpha 2c ( IFN-alpha 2c) by metal chelate affinity chromatography, N-terminal 6-histidine tag was introduced via genetic manipulation. Two preparations of IFN material were purified; one contained IFN-alpha 2c with the 6-histidine tag, and the other contained IFN-alpha 2c without the 6-histidine tag. The antigenic properties of the human IFN-alpha 2c subvariant with and without the 6-histidine tag, as well as the effects of the N-terminal 6-histidine tag on IFN-alpha 2c interaction with the extracellular domain of human IFN-alpha receptor chain 2 ( IFNAR2-EC) were examined. For the purposes of this study, IFNs were characterized by Western blots with anti-IFN monoclonal antibodies ( mAb) and bioassays. Immunoblot analyses showed differences between IFN-alpha 2c-6-histidine tag and IFN-alpha 2a, b, c in their interaction with IFNAR2-EC. We also observed differences between IFN-alpha 2c-6-histidine tag and IFN-alpha 2a, b, c in bioactivities. This study is the first report that shows that an N-terminal 6-histidine tag on IFN-alpha 2c can affect its interaction with receptor and cause a different bioactivity.

    See More

  1. Keywords:

External Sources

  1. View record in Web of Science®
  2. WOS: 000243076600003

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel