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Visualization and identification of IL-7 producing cells in reporter mice

  1. Author:
    Mazzucchelli, R. I.
    Warming, S.
    Lawrence, S. M.
    Ishii, M.
    Abshari, M.
    Washington, A. V.
    Feigenbaum, L.
    Warner, A. C.
    Sims, D. J.
    Li, W. Q.
    Hixon, J. A.
    Gray, D. H.
    Rich, B. E.
    Morrow, M.
    Anver, M. R.
    Cherry, J.
    Naf, D.
    Sternberg, L. R.
    McVicar, D. W.
    Farr, A. G.
    Germain, R. N.
    Rogers, K.
    Jenkins, N. A.
    Copeland, N. G.
    Durum, S. K.

  2. Author Address

    Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, NIH, Frederick, MD, USA.
    1. Year: 2009
    2. Epub Date: 11/13/2009
  2. Journal: Plos One
    1. 4
    2. 11
    3. Pages: e7637
  4. Type of Article: Article
  5. ISSN: 1932-6203 (Electronic);1932-6203 (Linking)
  1. Abstract:

    Interleukin-7 (IL-7) is required for lymphocyte development and homeostasis although the actual sites of IL-7 production have never been clearly identified. We produced a bacterial artificial chromosome (BAC) transgenic mouse expressing ECFP in the Il7 locus. The construct lacked a signal peptide and ECFP (enhanced cyan fluorescent protein) accumulated inside IL-7-producing stromal cells in thoracic thymus, cervical thymus and bone marrow. In thymus, an extensive reticular network of IL-7-containing processes extended from cortical and medullary epithelial cells, closely contacting thymocytes. Central memory CD8 T cells, which require IL-7 and home to bone marrow, physically associated with IL-7-producing cells as we demonstrate by intravital imaging.

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  2. DOI: 10.1371/journal.pone.0007637
  3. PMID: 19907640
  4. PMCID: PMC2770321

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