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Non-AUG translational initiation of a short CAPC transcript generating protein isoform

  1. Author:
    Anaganti, S.
    Hansen, J. K.
    Ha, D.
    Hahn, Y.
    Chertov, O.
    Pastan, I.
    Bera, T. K.

  2. Author Address

    Anaganti, Suresh, Hansen, Johanna K.; Ha, Duc, Hahn, Yoonsoo, Pastan, Ira, Bera, Tapan K.] NCI, Mol Biol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Chertov, Oleg] SAIC Frederick Inc, NCI, Adv Technol Program, Prot Chem Lab, Frederick, MD 21702 USA.
    1. Year: 2009
  2. Journal: Biochemical and Biophysical Research Communications
    1. 380
    2. 3
    3. Pages: 508-513
  4. Type of Article: Article
  1. Abstract:

    CAPC (also known as LRRC26) is a new gene with restricted expression in normal tissues, and with expression in many cancers and cancer cell lines. We have identified and characterized a short-transcript of CAPC(S-CAPC). The nucleotide sequence analysis of CAPC mRNA showed that the transcription for S-CAPC starts at position +610 on the L-CAPC transcript. Interestingly, no translation initiation codon 'AUG' is present in this transcript. To determine if a non-AUG start site is utilized, the S-CAPC sequence was cloned into an expression vector With C-terminal myc and histidine tags, and transfected into 293T cells. Western blot and MALDI-TOF MS analysis on purified S-CAPC gave two distinct peaks at approximately 7.5 kDa. N-terminal amino acid sequencing of the purified 7.5 kDa protein product indicated that translation starts at the codon for cysteine on the S-CAPC transcript generating a 7.5 kDa CAPC Protein products translated from a non-AUG initiation site. Published by Elsevier Inc

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External Sources

  1. PMID: 19250639

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