Skip NavigationSkip to Content
Return Return to Search Results

Characterization and expression analysis of type I interferon in common carp Cyprinus carpio L

  1. Author:
    Kitao, Y.
    Kono, T.
    Korenaga, H.
    Lizasa, T.
    Nakamura, K.
    Savan, R.
    Sakai, M.

  2. Author Address

    Kitao, Yoichi, Kono, Tomoya, Korenaga, Hiroki, Lizasa, Toshiyuki, Nakamura, Keisuke, Sakai, Masahiro] Miyazaki Univ, Fac Agr, Miyazaki 8892192, Japan. [Savan, Ram] NCI, Ctr Canc Res, Expt Immunol Lab, Frederick, MD 21702 USA.
    1. Year: 2009
  2. Journal: Molecular Immunology
    1. 46
    2. 13
    3. Pages: 2548-2556
  4. Type of Article: Article
  1. Abstract:

    The type I interferon (I-IFN) gene has recently been cloned and sequenced in the common carp species Cyprinus carpio L Carp I-IFN cDNA is composed of 675 base pairs and is translated into a protein of 186 amino acid residues. The carp I-IFN encodes a predicted signal peptide of 23 amino acid residues and contains the I-IFN family signature His(140)-Trp(158). Analysis of the homology between carp I-IFN and other known I-IFN and type II interferon (II-IFN) family members has revealed significant similarities to grass carp I-IFN. Phylogenetic analysis demonstrated that carp I-IFN clusters with I-IFN in teleosts, away from the other II-IFN family members. In addition, the gene structure for carp I-IFN is composed of 5 exons and 4 introns, a composition that is similar to that of the teleost I-IFN gene. RT-PCR analysis did not reveal gene expression in un-stimulated tissues including intestine, liver, gill, head kidney, muscle, spleen, mid-kidney and skin. However, I-IFN expression levels increased following stimulation with imiquimod in the head kidney cells. Furthermore, recombinant carp I-IFN protein (mature form) produced via the cell-free protein synthesis system stimulated the expression of the interferon-inducible Mx gene in the head kidney cells. (C) 2009 Elsevier Ltd. All rights reserved.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1016/j.molimm.2009.05.012
  3. PMID: 19520432

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel