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Alternative transcripts expressed by small bristles, the Drosophila melanogaster nxf1 gene

  1. Author:
    Ivankova, N.
    Tretyakova, I.
    Lyozin, G. T.
    Avanesyan, E.
    Zolotukhin, A.
    Zatsepina, O. G.
    Evgen'ev, M. B.
    Mamon, L. A.

  2. Author Address

    [Ivankova, Natalia; Tretyakova, Irina; Avanesyan, Elina; Mamon, Ludmila A.] St Petersburg State Univ, Dept Genet & Breeding, St Petersburg, Russia. [Lyozin, George T.; Evgen'ev, Michael B.] Cell Biophys Inst, Pushchino, Russia. [Zolotukhin, Andrei] Natl Canc Inst Frederick, NIH, Frederick, MD USA. [Zatsepina, Olga G.; Evgen'ev, Michael B.] VA Engelhardt Mol Biol Inst, Moscow 117984, Russia.;Mamon, LA, St Petersburg State Univ, Dept Genet & Breeding, St Petersburg, Russia.;mamon@LM2010.spb.edu
    1. Year: 2010
    2. Date: Jun
  2. Journal: Gene
    1. 458
    2. 1-2
    3. Pages: 11-19
  4. Type of Article: Article
  5. ISSN: 0378-1119
  1. Abstract:

    The tissue-specific accumulation of small bristles (Dm nxf1) transcripts at different developmental stages of Drosophila melanogaster was analyzed by Northern blots and RT PCR. We identified four distinct transcripts: ubiquitous (3.5 kb); ovary and early embryo specific (3.3 kb); testis specific (1.9 kb and 2.8 kb) and nervous system specific (5.1 kb). The pattern of Dm nxf1 gene expression in ovaries and early embryos (0-2 h) is similar: the sizes of transcripts range from 3.0 to 3.5 kb. We propose that this size variability may reflect the different extent of cytoplasmic polyadenylation. In testes, the 2.8-kb transcript originates from alternative termination of transcription and the 1.9-kb transcript is supposed to originate from an alternative transcription start. During ontogenesis, the 5.1-kb transcript can be clearly detected in 10- to 18-h-old embryos, most prominently in the nervous ganglia of larvae, and it represents a major species in imago head extracts. We found that the 5.1-kb transcript, similarly to the nxf1 heavy transcripts in Homo sapiens and Mus musculus, results from the retention of intron 5-6 that corresponds to the intron 10-11 in Hs nxf1 and Mm nxf1 genes. (C) 2010 Elsevier B.V. All rights reserved.

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External Sources

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  2. DOI: 10.1016/j.gene.2010.02.013
  3. View record in Web of Science®
  4. WOS: 000278305000002

Library Notes

  1. Fiscal Year: FY2009-2010
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