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Self assembled bi-functional peptide hydrogels with biomineralization-directing peptides

  1. Author:
    Gungormus, M.
    Branco, M.
    Fong, H.
    Schneider, J. P.
    Tamerler, C.
    Sarikaya, M.

  2. Author Address

    [Gungormus, Mustafa; Fong, Hanson; Tamerler, Candan; Sarikaya, Mehmet] Univ Washington, Seattle, WA 98195 USA. [Branco, Monica; Schneider, Joel P.] NCI, Ctr Canc Res, Biol Chem Lab, Frederick, MD 21701 USA.;Sarikaya, M, Univ Washington, Seattle, WA 98195 USA.;musgun@u.washington.edu monica.branco@nih.gov hfong@u.washington.edu Joel.Schneider@nih.gov Candan@u.washington.edu sarikaya@u.washington.edu
    1. Year: 2010
    2. Date: Oct
  2. Journal: Biomaterials
    1. 31
    2. 28
    3. Pages: 7266-7274
  4. Type of Article: Article
  5. ISSN: 0142-9612
  1. Abstract:

    A peptide-based hydrogel has been designed that directs the formation of hydroxyapatite. MDG1, a twenty-seven residue peptide, undergoes triggered folding to form an unsymmetrical beta-hairpin that self-assembles in response to an increase in solution ionic strength to yield a mechanically rigid, self supporting hydrogel. The C-terminal portion of MDG1 contains a heptapeptide (MLPHHGA) capable of directing the mineralization process. Circular dichroism spectroscopy indicates that the peptide folds and assembles to form a hydrogel network rich in beta-sheet secondary structure. Oscillatory rheology indicates that the hydrogel is mechanically rigid (G'(similar to)-2500 Pa) before mineralization. In separate experiments, mineralization was induced both biochemically and with cementoblast cells. Mineralization-domain had little effect on the mechanical rigidity of the gel. SEM and EDXS show that MDG1 gels are capable of directing the formation of hydroxapatite. Control hydrogels, prepared by peptides either lacking the mineral-directing portion or reversing its sequence, indicated that the heptapeptide is necessary and its actions are sequence specific. Published by Elsevier Ltd.

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External Sources

  1. View Full Text
  2. DOI: 10.1016/j.biomaterials.2010.06.010
  3. View record in Web of Science®
  4. WOS: 000281183000007

Library Notes

  1. Fiscal Year: FY2010-2011
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