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BUD22 Affects Ty1 Retrotransposition and Ribosome Biogenesis in Saccharomyces cerevisiae

  1. Author:
    Dakshinamurthy, A.
    Nyswaner, K. M.
    Farabaugh, P. J.
    Garfinkel, D. J.

  2. Author Address

    [Dakshinamurthy, Arun; Nyswaner, Katherine M.; Garfinkel, David J.] NCI, Gene Regulat & Chromosome Biol Lab, Ctr Canc Res, Frederick, MD 21702 USA. [Dakshinamurthy, Arun; Farabaugh, Philip J.] Univ Maryland, Dept Biol Sci, Program Mol & Cell Biol, Baltimore, MD 21250 USA.;Garfinkel, DJ, Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA.;djgarf@bmb.uga.edu
    1. Year: 2010
    2. Date: Aug
  2. Journal: Genetics
    1. 185
    2. 4
    3. Pages: 1193-U99
  4. Type of Article: Article
  5. ISSN: 0016-6731
  1. Abstract:

    A variety of cellular factors affect the movement of the retrovirus-like transposon Ty1. To identify genes involved in Ty1 virus-like particle (VLP) function, the level of the major capsid protein (Gag-p45) and its proteolytic precursor (Gag-p49p) was monitored in a subset of Ty1 cofactor mutants. Twenty-nine of 87 mutants contained alterations in the level of Gag; however, only bud22 Delta showed a striking defect in Gag processing. BUD22 affected the +1 translational frameshifting event required to express the Pol proteins protease, integrase, and reverse transcriptase. Therefore, it is possible that the bud22 Delta mutant may not produce enough functional Ty1 protease to completely process Gag-p49 to p45. Furthermore, BUD22 is required for 18S rRNA processing and 40S subunit biogenesis and influences polysome density. Together our results suggest that BUD22 is involved in a step in ribosome biogenesis that not only affects general translation, but also may alter the frameshifting efficiency of ribosomes, an event central to Ty1 retrotransposition.

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External Sources

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  2. DOI: 10.1534/genetics.110.119115
  3. View record in Web of Science®
  4. WOS: 000281907700006

Library Notes

  1. Fiscal Year: FY2009-2010
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