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Pitx2 is an upstream activator of extraocular myogenesis and survival

  1. Author:
    Zacharias, A. L.
    Lewandoski, M.
    Rudnicki, M. A.
    Gage, P. J.

  2. Author Address

    [Zacharias, Amanda L.; Gage, Philip J.] Univ Michigan, Sch Med, Dept Ophthalmol & Visual Sci, Ann Arbor, MI 48105 USA. [Gage, Philip J.] Univ Michigan, Sch Med, Dept Cell & Dev Biol, Ann Arbor, MI 48105 USA. [Lewandoski, Mark] NCI, Canc & Dev Biol Lab, Ft Detrick, MD 21702 USA. [Rudnicki, Michael A.] Ottawa Hosp, Res Inst, Regenerat Med Program, Sprott Ctr Stem Cell Res, Ottawa, ON K1H 8L6, Canada. [Rudnicki, Michael A.] Univ Ottawa, Dept Med, Ottawa, ON K1H 8L6, Canada.;Gage, PJ, Univ Michigan, Sch Med, Dept Ophthalmol & Visual Sci, Ann Arbor, MI 48105 USA.;philgage@umich.edu
    1. Year: 2011
    2. Date: Jan
  2. Journal: Developmental Biology
    1. 349
    2. 2
    3. Pages: 395-405
  4. Type of Article: Article
  5. ISSN: 0012-1606
  1. Abstract:

    The transcription factors required to initiate myogenesis in branchial arch- and somite-derived muscles are known, but the comparable upstream factors required during extraocular muscle development have not been identified. We show Pax7 is dispensable for extraocular muscle formation, whereas Pitx2 is cell-autonomously required to prevent apoptosis of the extraocular muscle primordia. The survival requirement for Pitx2 is stage-dependent and ends following stable activation of genes for the muscle regulatory factors (e.g. Myf5,MyoD), which is reduced in the absence of Pitx2. Further, PITX2 binds and activates transcription of the Myf5 and MyoD promoters, indicating these genes are direct targets. Collectively, these data demonstrate that PITX2 is required at several steps in the development of extraocular muscles, acting first as an anti-apoptotic factor in pre-myogenic mesoderm, and subsequently to activate the myogenic program in these cells. Thus, Pitx2 is the first demonstrated upstream activator of myogenesis in the extraocular muscles. (C) 2010 Elsevier Inc. All rights reserved.

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External Sources

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  2. DOI: 10.1016/j.ydbio.2010.10.028
  3. View record in Web of Science®
  4. WOS: 000286412800027

Library Notes

  1. Fiscal Year: FY2010-2011
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