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Biacore For Macromolecular Interaction

  1. Author:
    Fivash, M.
    Towler, E. M.
    Fisher, R. J.
    1. Year: 1998
  2. Journal: Current Opinion in Biotechnology
    1. 9
    2. 1
    3. Pages: 97-101
  4. Type of Article: Review
  1. Abstract:

    Examination of the literature for the period of this review revealed nearly two hundred citations that employed surface plasmon resonance (SPR) spectroscopy using BIAcore technology to evaluate biospecific interactions, demonstrating the increasing popularity of this powerful technique. Among these we noted the development of several new applications/modifications of standard techniques. In general, we find the qualitative aspects of the reported experiments to be excellent but the quantitative descriptions (k(T), k(on), k(off), and K-eq) as well as the binding models still lagging behind. (C) Current Biology Ltd ISSN 0958-1669. [References: 34]

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