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Comparison of the Quantiplex version 3.0 assay and a sensitized Amplicor Monitor assay for measurement of human immunodeficiency virus type 1 RNA levels in plasma samples

  1. Author:
    Highbarger, H. C.
    Alvord, W. G.
    Jiang, M. K.
    Shah, A. S.
    Metcalf, J. A.
    Lane, H. C.
    Dewar, R. L.

  2. Author Address

    Dewar RL NCI, Frederick Canc Res & Dev Ctr, Virus Isolat Lab, Clin Serv Prog,SAIC Frederick Frederick, MD 21702 USA NCI, Frederick Canc Res & Dev Ctr, Virus Isolat Lab, Clin Serv Prog,SAIC Frederick Frederick, MD 21702 USA Data Management Serv Inc Frederick, MD USA NCI, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA NIAID, Immunoregulat Lab Bethesda, MD 20892 USA
    1. Year: 1999
  2. Journal: Journal of Clinical Microbiology
    1. 37
    2. 11
    3. Pages: 3612-3614
  4. Type of Article: Article
  1. Abstract:

    This study evaluated correlation and agreement between version 3 of the Quantiplex human immunodeficiency virus type 1 (HIV-1) RNA assay (v3 branched DNA [bDNA]) and a sensitized Amplicor HIV-1 Monitor assay (reverse transcription [RT]-PCR) for the measurement of HIV RNA. Three hundred eighteen samples from 59 randomly selected, HIV-1-seropositive persons on various drug protocols from the National Institute of Allergy and Infectious Diseases HIV outpatient clinic were studied. The results indicate that v3 bDNA and RT-PCR are highly correlated (r = 0.98) and are in good agreement (mean difference in log(10) copies/ml +/- 2 standard deviations = 0.072 +/- 0.371). The relationship between values obtained by both assays is given by the following equation: log(10)v3 bDNA = -0.0915 + 1.0052.log(10)RT-PCR This represents a 1.026-fold difference between log(10)RT-PCR values and log(10)v3 bDNA values. [References: 11]

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