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Two distinct forms of the 64,000 M-r protein of the cleavage stimulation factor are expressed in mouse male germ cells

  1. Author:
    Wallace, A. M.
    Dass, B.
    Ravnik, S. E.
    Tonk, V.
    Jenkins, N. A.
    Gilbert, D. J.
    Copeland, N. G.
    MacDonald, C. C.

  2. Author Address

    MacDonald CC Texas Tech Univ, Hlth Sci Ctr, Dept Cell Biol & Biochem, Ctr Med 3601 4th St Lubbock, TX 79430 USA Texas Tech Univ, Hlth Sci Ctr, Dept Cell Biol & Biochem, Ctr Med Lubbock, TX 79430 USA Texas Tech Univ, Hlth Sci Ctr, Dept Pediat & Pathol, Ctr Med Lubbock, TX 79430 USA Texas Tech Univ, Hlth Sci Ctr, SW Canc Ctr, Ctr Med Lubbock, TX 79430 USA NCI, Frederick Canc Res & Dev Ctr, Mammalian Genet Lab, Adv Biosci Labs,Basic Res Program Frederick, MD 21702 USA
    1. Year: 1999
  2. Journal: Proceedings of the National Academy of Sciences of the United States of America
    1. 96
    2. 12
    3. Pages: 6763-6768
  4. Type of Article: Article
  1. Abstract:

    Polyadenylation in male germ cells differs from that in somatic cells, Many germ cell mRNAs do not contain the canonical AAUAAA in their 3' ends but are efficiently polyadenylated. To determine whether the 64,000 M-r protein of the cleavage stimulation factor (CstF-64) is altered in male germ cells, we examined its expression in mouse testis, In addition to the 64,000 M-r form, we found a related approximate to 70,000 M-r protein that is abundant in testis, at low levels in brain, and undetectable in all other tissues examined. Expression of the approximate to 70,000 M-r CstF-64 was limited to meiotic spermatocytes and postmeiotic spermatids in testis, In contrast, the 64,000 M-r form was absent from spermatocytes, suggesting that the testis-specific CstF-64 might control expression of meiosis-specific genes. To determine why the 64,000 M-r CstF-64 is not expressed in spermatocytes, we mapped its chromosomal location to the X chromosome in both mouse and human. CstF-64 may, therefore, be absent in spermatocytes because the X chromosome is inactivated during male meiosis, By extension, the testis-specific CstF-64 may be expressed from an autosomal homolog of the X chromosomal gene. [References: 92]

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