Skip NavigationSkip to Content
Return Return to Search Results

Emi2 Is Essential for Mouse Spermatogenesis

  1. Author:
    Gopinathan, Lakshmi
    Szmyd, Radoslaw
    Low, Diana
    Diril, M Kasim
    Chang, Heng-Yu
    Coppola, Vincenzo
    Liu, Kui
    Tessarollo, Lino
    Guccione, Ernesto
    van Pelt, Ans M M
    Kaldis, Philipp

  2. Author Address

    Institute of Molecular and Cell Biology (IMCB), A(*)STAR (Agency for Science, Technology and Research), 61 Biopolis Drive, Proteos #3-09, Singapore 138673, Republic of Singapore., Institute of Molecular and Cell Biology (IMCB), A(*)STAR (Agency for Science, Technology and Research), 61 Biopolis Drive, Proteos #3-09, Singapore 138673, Republic of Singapore; NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore (NUS), Singapore 117456, Republic of Singapore., Department of Biochemistry and Molecular Cell Biology, School of Medicine, Taipei Medical University, Taipei 11031, Taiwan., Mouse Cancer Genetics Program, National Cancer Institute, NCI-Frederick, Building 560, 1050 Boyles Street, Frederick, MD 21702-1201, USA., Department of Chemistry and Molecular Biology, University of Gothenburg, SE-405 30 Gothenburg, Sweden., Institute of Molecular and Cell Biology (IMCB), A(*)STAR (Agency for Science, Technology and Research), 61 Biopolis Drive, Proteos #3-09, Singapore 138673, Republic of Singapore; Department of Biochemistry, National University of Singapore (NUS), Singapore 117597, Republic of Singapore., Center for Reproductive Medicine, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, the Netherlands., Institute of Molecular and Cell Biology (IMCB), A(*)STAR (Agency for Science, Technology and Research), 61 Biopolis Drive, Proteos #3-09, Singapore 138673, Republic of Singapore; Department of Biochemistry, National University of Singapore (NUS), Singapore 117597, Republic of Singapore. Electronic address: kaldis@imcb.a-star.edu.sg.,
    1. Year: 2017
    2. Date: Jul 18
  2. Journal: Cell Reports
    1. 20
    2. 3
    3. Pages: 697-708
  4. Type of Article: Article
  1. Abstract:

    The meiotic functions of Emi2, an inhibitor of the APC/C complex, have been best characterized in oocytes where it mediates metaphase II arrest as a component of the cytostatic factor. We generated knockout mice to determine the in vivo functions of Emi2-in particular, its functions in the testis, where Emi2 is expressed at high levels. Male and female Emi2 knockout mice are viable but sterile, indicating that Emi2 is essential for meiosis but dispensable for embryonic development and mitotic cell divisions. We found that, besides regulating cell-cycle arrest in mouse eggs, Emi2 is essential for meiosis I progression in spermatocytes. In the absence of Emi2, spermatocytes arrest in early diplotene of prophase I. This arrest is associated with decreased Cdk1 activity and was partially rescued by a knockin mouse model of elevated Cdk1 activity. Additionally, we detected expression of Emi2 in spermatids and sperm, suggesting potential post-meiotic functions for Emi2. Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1016/j.celrep.2017.06.033
  3. PMID: 28723571
  4. View record in Web of Science®
  5. WOS: 000405690100015

Library Notes

  1. Fiscal Year: FY2016-2017
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel