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The p16/Cdkn2a/Ink4a gene is frequently deleted in nitrosourea-induced rat glial tumors

  1. Author:
    Schlegel, J.
    Piontek, G.
    Kersting, M.
    Schuermann, M.
    Kappler, R.
    Scherthan, H.
    Weghorst, C.
    Buzard, G.
    Mennel, H. D.

  2. Author Address

    Schlegel J Inst Pathol, Neuropathol Lab Ismaninger Str 22 D-81675 Munich Germany Univ Med Ctr, Dept Neuropathol, Mol Neurobiol Lab Marburg Germany Univ Med Ctr, Dept Hematol & Oncol Marburg Germany Univ Kaiserslautern, Dept Human Biol & Human Genet D-67663 Kaiserslautern Germany Ohio State Univ, Coll Med & Publ Hlth, Sch Publ Hlth, Div Environm Hlth Sci Columbus, OH 43210 USA NCI, Intramural Res Support Program, SAIC Frederick, FCRDC Frederick, MD 21701 USA
    1. Year: 1999
  2. Journal: Pathobiology
    1. 67
    2. 4
    3. Pages: 202-206
  4. Type of Article: Article
  1. Abstract:

    The present study investigates nitrosourea-induced rat (Rattus norvegicus) glioma cell lines for the functional status of the p16/Cdkn2a/Ink4a gene, which encodes the p16 cdk4 inhibitor and the alternative reading frame protein, p19ARF. We detected homozygous deletions of the p16/Cdkn2a/Ink4a gene locus in 4 of 5 glioma cell lines (C6, F98, RG2, and RGL.3), but not in the 9L gliosarcoma cell line or in a rat primary fibroblast cell line. RT-PCR demonstrated expression of the p16 and p19ARF mRNAs only in 9L cells and in rat fibroblasts. Comparative genomic in situ hybridization showed that the copy number of rat chromosome RNO5 was not altered in any of the glioma cell lines investigated, indicating that the deletions result from a discrete loss in the region of the p16/Cdkn2a/Ink4a locus. This is the first report of p16/Cdkn2a/Ink4a deletions present in nitrosourea-induced rat glioma cell lines. Since this genetic alteration is also commonly observed in human malignant glial tumors, our results validate the use of chemically induced rat glioma cell lines as an experimental model in the development of gene therapy strategies. Copyright (C) 2000 S. Karger AG, Basel. [References: 24]

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