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Nitrogen-starvation triggers cellular accumulation of triacylglycerol in Metarhizium robertsii

  1. Author:
    Chen, Yixiong
    Cen, Kai
    Lu, Yuzhen
    Zhang, Siwei
    Shang, Yanfang
    Wang, Chengshu

  2. Author Address

    Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Key Lab Insect Dev & Evolutionary Biol, Shanghai 200032, Peoples R China.NCI, RNA Biol Lab, NIH, Frederick, MD 21702 USA.
    1. Year: 2018
    2. Date: Jun
  2. Journal: FUNGAL BIOLOGY
  3. ELSEVIER SCI LTD,
    1. 122
    2. 6
    3. Pages: 410-419
  5. Type of Article: Article
  6. ISSN: 1878-6146
  1. Abstract:

    Nitrogen starvation can induce cellular triacylglycerol (TAG) accumulation in different organisms with an unclear mechanism. In this study, we performed nutrient starvation and lipid droplet (LD) proteomics analyses of the filamentous fungus Metarhizium robertsii. Our results indicated that nitrogen starvation activated cell autophagic activity but inhibited the internalization of LDs into vacuoles for degradation. LD proteomic analyses identified an array of differentially accumulated proteins including autophagyrelated (ATG) proteins, heat shock proteins, TAG metabolic and phospholipid biosynthetic enzymes when the fungus was grown in different nutrient conditions. In contrast to the highly activated MrATG8, the ATG proteins involved in vacuolar LD internalization were down-regulated after nitrogen starvation. Cellular TAG contents were increased in different ATG-gene null mutants of M. robertsii. In addition, TAG increase could be due to the up-regulation of TAG biogenesis along with the down-regulation of TAG catabolic enzymes in fungal cells after nitrogen deprivation. The data of this study benefit our understanding of the mechanism of nitrogen starvation induced TAG increase in different cells. (C) 2017 British Mycological Society. Published by Elsevier Ltd. All rights reserved.

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External Sources

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  2. DOI: 10.1016/j.funbio.2017.07.001
  3. PMID: 29801784
  4. View record in Web of Science®
  5. WOS: 000436200900004

Library Notes

  1. Fiscal Year: FY2017-2018
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