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Physical assignments of human chromosome 13 genes on pig chromosome 11 demonstrate extensive synteny and gene order conservation between pig and human

  1. Author:
    Sun, H. S.
    Yerle, M.
    Pinton, P.
    Chardon, P.
    Rogel-Gaillard, C.
    Lyons, L. A.
    Laughlin, T. F.
    Tuggle, C. K.

  2. Author Address

    Tuggle CK Iowa State Univ, Dept Anim Sci Ames, IA 50011 USA Iowa State Univ, Dept Anim Sci Ames, IA 50011 USA INRA, Lab Genet Cellulaire F-31326 Castanet Tolosan France NCI, Lab Genomic Divers Bethesda, MD 20892 USA NCI, FCRDC, Biol Carcinogenesis & Dev Program, SAIC Frederick Bethesda, MD 20892 USA
    1. Year: 1999
  2. Journal: Animal Genetics
    1. 30
    2. 4
    3. Pages: 304-308
  4. Type of Article: Article
  1. Abstract:

    Previous mapping between the human and pig genomes suggested extensive conservation of human chromosome 13 (HSA13) to pig chromosome 11 (SSC11). The objectives of this study were comparative gene mapping of pig homologs of HSA13 genes and examining gene order within this conserved synteny group by physical assignment of each locus. A detailed HSA13 to SSC11 comparison was chosen since the comparative gene map is not well developed for these chromosomes and a rearranged gene order within conserved synteny groups was observed from the comparison between HSA13 and bovine chromosome 12 (BTA12). Heterologous primers for PCR were designed and used to amplify pig homologous fragments. The pig fragments were sequenced to confirm the homology. Six pig STSs (FLT1, ESD, RB1, HTR2A, EDNRB, and F10) were physically mapped using a somatic cell hybrid panel to Department of Animal SSC11, and fluorescent in situ hybridization (FISH) mapping was also applied to improve map resolution and determine gene order. Results from this study increase the comparative information available on SSC11 and suggest a conserved gene order on SSC11 and HSA13, in Department of Animal contrast to human:bovine comparisons of this syntenic group. [References: 28]

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