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Heterologous expression of murine lymphotoxins in Escherichia coli and the preparation of antibodies

  1. Author:
    Korobko, V. G.
    Boichenko, V. E.
    Kuprash, D. V.
    Turetskaya, R. L.
    Nedospasov, S. A.

  2. Author Address

    Nedospasov SA Russian Acad Sci, VA Engelhardt Mol Biol Inst Ul Vavilova 32 Moscow 117984 Russia Russian Acad Sci, VA Engelhardt Mol Biol Inst Moscow 117984 Russia Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem Moscow 117871 Russia Moscow MV Lomonosov State Univ, Belozersky Inst Physicochem Biol Moscow 119899 Russia NIH Frederick, MD 21702 USA
    1. Year: 1999
  2. Journal: Bioorganicheskaia Khimiia
    1. 25
    2. 4
    3. Pages: 270-274
  4. Type of Article: Article
  1. Abstract:

    Genes encoding fragments of polypeptide chains of murine lymphotoxins (LT), namely, LT-alpha truncated from the N-terminus and the LT-beta extracellular domain, containing N-terminal hepta- and hexahistidine epitopes, respectively, were expressed in E. coli cells. The recombinant proteins purified by metallochelate chromatography were used to obtain polyclonal antibodies that specifically recognize murine LT. [References: 23]

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