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A near-infrared light-mediated cleavable linker strategy using the heptamethine cyanine chromophore

  1. Author:
    Luciano,Michael
    Nourian, Saghar
    Gorka, Alexander P
    Nani, Roger R
    Nagaya, Tadanobu
    Kobayashi, Hisataka
    Schnermann,Martin

  2. Author Address

    Chemical Biology Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, United States., Laboratory of Molecular Theranostics, NIH/NCI/CCR, Bethesda, MD, United States., Chemical Biology Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, United States. Electronic address: schnermannmj@mail.nih.gov.,
    1. Year: 2020
    2. Epub Date: 2020 06 15
  2. Journal: Methods in enzymology
    1. 641
    2. Pages: 245-275
  4. Type of Article: Article
  5. ISSN: 0076-6879
  1. Abstract:

    Optical methods offer the potential to manipulate living biological systems with exceptional spatial and temporal control. Caging bioactive molecules with photocleavable functional groups is an important strategy that could be applied to a range of problems, including the targeted delivery of otherwise toxic therapeutics. However existing approaches that require UV or blue light are difficult to apply in organismal settings due to issues of tissue penetration and light toxicity. Photocaging groups built on the heptamethine cyanine scaffold enable the targeted delivery of bioactive molecules using near-IR light (up to 780nm) in live animal settings. Here we provide a detailed procedure demonstrating the utility of the heptamethine cyanine caging group to create a light-cleavable linker between an antibody, panitumumab, and a therapeutic small molecule in the duocarmycin class of natural products. Descriptions of the design and synthesis of the small molecule component, assembly of the antibody conjugate, in vitro analysis of uncaging, in vivo imaging, and impact on tumor progression are provided. © 2020 Elsevier Inc. All rights reserved.

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External Sources

  1. View Full Text
  2. DOI: 10.1016/bs.mie.2020.04.043
  3. PMID: 32713525
  4. View record in Web of Science®
  5. WOS: 000556316000012
  6. PII : S0076-6879(20)30171-3

Library Notes

  1. Fiscal Year: FY2019-2020
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