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A simple colorimetric experiment using mammalian cell culture to study metabolism

  1. Author:
    Sharma, Kanika
    Fizet, Kiel J
    Montgomery, Kathlyene R
    Smeltzer, Nathan A
    Sikorski, Madison H
    Brown, Kennedy G
    Beyke, Brandon J
    Burkhart, Ryan C
    Lynn, Abigail N
    Grandinetti, Giovanna [ORCID]

  2. Author Address

    NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA., Department of Chemistry, Muskingum University, New Concord, Ohio, USA., Department of Biology, Muskingum University, New Concord, Ohio, USA.,
    1. Year: 2020
    2. Date: Sep 17
    3. Epub Date: 2020 09 17
  2. Journal: Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology
  4. Type of Article: Article
  5. ISSN: 1470-8175
  1. Abstract:

    The goal of this laboratory exercise is to give upper-level undergraduate students an introduction to sterile technique in mammalian cell culture and metabolism. The experiment can be completed within a 3-h lab period and can be performed either in conjunction with other biochemistry/metabolism experiments or used as a stand-alone experiment. In this experiment, students are tasked with relating the acidification of cell culture medium to metabolism in order to elucidate the mechanism of action for a compound. Students can relate their experimental results to topics covered on glycolysis and oxidative phosphorylation in upper-level biochemistry classes as well as gain valuable experience relating metabolism to drug discovery. © 2020 International Union of Biochemistry and Molecular Biology.

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External Sources

  1. View Full Text
  2. DOI: 10.1002/bmb.21457
  3. PMID: 32942341
  4. View record in Web of Science®
  5. WOS: 000569981000001

Library Notes

  1. Fiscal Year: FY2020-2021
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