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Visualizing RNA Cytidine Acetyltransferase Activity by Northern Blotting

  1. Author:
    Bryson, Keri M
    Thalalla Gamage,Supuni
    Meier,Jordan

  2. Author Address

    Chemical Biology Laboratory, National Cancer Institute, Frederick, Maryland.,
    1. Year: 2020
    2. Date: Dec
  2. Journal: Current protocols in chemical biology
    1. 12
    2. 4
    3. Pages: e89
  4. Type of Article: Article
  5. Article Number: e89
  1. Abstract:

    Cytidine acetyltransferases are an emerging class of nucleic-acid-modifying enzymes responsible for the establishment of N4 -acetylcytidine (ac4C) in RNA. In contrast to histone acetyltransferases, whose activity is commonly studied by western blotting, relatively few methods exist for quickly assessing the activity of cytidine acetyltransferases from a biological sample of interest or the distribution of ac4C across different RNA species. In this protocol, we describe a method for analysis of cellular cytidine acetyltransferase activity using dot- and immuno-northern-blotting-based detection. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Detection of N4 -Acetylcytidine in RNA by dot blotting Basic Protocol 2: Visualizing N4 -Acetylcytidine Distribution in RNA by northern blotting. © 2020 Wiley Periodicals LLC.

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External Sources

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  2. DOI: 10.1002/cpch.89
  3. PMID: 33275333

Library Notes

  1. Fiscal Year: FY2020-2021
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