Skip NavigationSkip to Content
Return Return to Search Results

Functional characterization of the gene encoding RLIM, the corepressor of LIM homeodomain factors

  1. Author:
    Ostendorff, H. P.
    Bossenz, M.
    Mincheva, A.
    Copeland, N. G.
    Gilbert, D. J.
    Jenkins, N. A.
    Lichter, P.
    Bach, I.

  2. Author Address

    Univ Hamburg, Ctr Mol Neurobiol, Martinistr 85, D-20251 Hamburg, Germany. Univ Hamburg, Ctr Mol Neurobiol, D-20251 Hamburg, Germany. German Canc Res Ctr, D-69120 Heidelberg, Germany. NCI, Frederick Canc Res & Dev Ctr, ABL Basic Res Program, Frederick, MD 21702 USA.
    1. Year: 2000
  2. Journal: Genomics
    1. 69
    2. 1
    3. Pages: 120-130
  4. Type of Article: Article
  1. Abstract:

    RLIM is a RING H2 zinc finger protein that acts as a negative coregulator for LIM homeodomain transcription factors. We have isolated genomic A clones that cover the entire mouse RLIM- encoding Rnf12 gene. The Rnf12 gene encompasses 20 kb and consists of at least five exons and four introns. Several transcriptional start sites within a 24-bp region were mapped around 300 nt upstream of the translational start site. Rnf12- specific mRNA can be detected in many tissues as revealed by Northern blot analysis. Transient cotransfections reveal that the proximal Rnf12 promoter can be activated in vitro by ubiquitously and more restrictively expressed transcription factors, some of which are known mediators of signal transduction pathways, e.g., mammalian Kruppel-like transcription factors, Sox and ets-related proteins, and RBP-J. We isolated a cDNA encoding human RLIM, which is highly conserved with mouse and chick RLIM. By fluorescence in situ hybridization and interspecific backcross analysis, we have localized the Rnf12 gene to the central regions of mouse and human chromosome X. (C) 2000 Academic Press.

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel