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Evaluation of Real-Time In Vitro Invasive Phenotypes

  1. Author:
    Delosh,Rene
    Shoemaker, Robert H.

  2. Author Address

    Frederick Natl Lab Canc Res, Leidos Biomed Res, Frederick, MD 21701 USA.NCI, Canc Prevent Div, Bethesda, MD 20892 USA.
    1. Year: 2021
  2. Book Title: Metastasis
  3. Humana Press, Inc.
    1. 2294
    2. Pages: 165-180
  5. Type of Work: Book Chapter
  6. ISBN: 978-1-0716-1349-8
  1. Abstract:

    The methods described here provide a standardized process for assessing in vitro tumor cell migration and invasion in real time. The kinetic data generated under these standardized conditions are reproducible and characteristic of individual tumor cell lines. The complex kinetic features of the data can be analyzed using parameters modeled after pharmacokinetic data processing. Application of the method to the array of tumor types included in the National Cancer Institute's sixty cell line panel (NCI60) revealed distinct modes of invasion with some tumor cell lines utilizing a mesenchymal mode and generating information-rich kinetic profiles. Other cell lines utilized an amoeboid mode not suitable for detection with this method. The method described will be useful as a guide for tumor cell line selection and as a starting point in designing experiments probing migration and invasion.

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External Sources

  1. View Full Text
  2. DOI: 10.1007/978-1-0716-1350-4_12
  3. PMID: 33742401
  4. View record in Web of Science®
  5. WOS: 000707157900013

Library Notes

  1. Fiscal Year: FY2021-2022
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