Skip NavigationSkip to Content
Return Return to Search Results

Precise Characterization of KRAS4B Proteoforms by Combining Immunoprecipitation with Top-Down Mass Spectrometry

  1. Author:
    Adams, Lauren M.
    Dehart,Caroline
    Kelleher, Neil L.

  2. Author Address

    Northwestern Univ, Dept Mol Biosci, Evanston, IL 60208 USA.Frederick Natl Lab Canc Res, NCI RAS Initiat, Frederick, MD USA.Northwestern Univ, Prote Ctr Excellence, Evanston, IL USA.Northwestern Univ, Dept Chem, Evanston, IL USA.
    1. Year: 2021
  2. Book Title: Ras Activity and Signaling
  3. Humana Press, Inc.
    1. 2262
    2. Pages: 47-64
  5. Type of Work: Book Chapter
  6. ISBN: 978-1-0716-1189-0
  1. Abstract:

    The characterization of biologically relevant post-translational modifications (PTMs) on KRAS4B has historically been carried out through methodologies such as immunoblotting with PTM-specific antibodies or peptide-based proteomic methods. While these methods have the potential to identify a given PTM on KRAS4B, they are incapable of characterizing or distinguishing the different molecular forms or proteoforms of KRAS4B from those of related RAS isoforms. We present a method that combines immunoprecipitation of KRAS4B with top-down mass spectrometry (IP-TDMS), thus enabling the precise characterization of intact KRAS4B proteoforms. We provide detailed protocols for the IP, LC-MS/MS, and data analysis comprising a successful IP-TDMS assay in the contexts of cancer cell lines and tissue samples.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1007/978-1-0716-1190-6_3
  3. PMID: 33977470
  4. View record in Web of Science®
  5. WOS: 000706804700004

Library Notes

  1. Fiscal Year: FY2021-2022
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel