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Detection of integrated murine leukemia viruses in a mouse model of acute myeloid leukemia by fluorescence in situ hybridization combined with tyramide signal amplification

  1. Author:
    Acar, H.
    Copeland, N. G.
    Gilbert, D. J.
    Jenkins, N. A.
    Largaespada, D. A.

  2. Author Address

    Largaespada DA Univ Minnesota, Ctr Canc, Dept Genet Cell Biol & Dev Res Bldg,425 E River Rd Minneapolis, MN 55455 USA Univ Minnesota, Ctr Canc, Dept Genet Cell Biol & Dev Minneapolis, MN 55455 USA Selcuk Univ, Dept Med Genet Konya Turkey NCI, Frederick Canc Res & Dev Ctr, Mouse Canc Genet Program Frederick, MD USA
    1. Year: 2000
    2. Date: Aug
  2. Journal: Cancer Genetics and Cytogenetics
    1. 121
    2. 1
    3. Pages: 44-51
  4. Type of Article: Article
  1. Abstract:

    This study rt as undertaken to develop a reliable method to enumerate and map somatically acquired, clonal, murine leukemia virus (MuLV) proviral insertions in acute myeloid leukemia (AML) cells from the BXH-2 mouse strain. This was achieved by using fluorescence in situ hybridization combined with tyramide signal amplification (FISH-TSA) and an 8.8 kilobase pair (kb) full-length ecotropic MuLV or 2.0 kb MuLV envelope (env) gene probe. Two-color FISH was utilized combining chromosome-specific probes for regions near the telomere and/or centromere and the MuLV probes. The technique reliably detected germline and somatically acquired, tumor-specific, MuLV proviruses in BXH-2 AML cell lines. It rr as possible to readily verify homozygous insertions at endogenous ecotropic,MuLV loci, Emv1 (chromosome 5), Emv2 (chromosome 8) and a BXH-2 strain-specific locus (chromosome 11). This strategy also verified the presence of molecularly cloned proviral insertions within the mouse Nf1 gene and another locus on distal chromosome II, as well as on chromosome 7 and chromosome 9 in BXH-2 AML cell line B117. The technique rc as also used to detect several new tumor-specific, proviral insertions in BXH-2 AML cell lines. (C) 2000 Elsevier Science Inc, All rights reserved. [References: 22]

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  2. DOI: 10.1016/S0165-4608(00)00232-6
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