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Microinjected Cdna Encoding Jak2 Protein-Tyrosine Kinase Induces Dna Synthesis in Nih 3t3 Cells

  1. Author:
    Smith, M. R.
    Duhe, R. J.
    Liu, Y. L.
    Farrar, W. L.

  2. Author Address

    Duhe RJ NCI INTRAMURAL RES SUPPORT PROGRAM SCI APPLICAT INT CORP FREDERICK CANC RES & DEV CTR POB B FREDERICK, MD 21702 USA NCI INTRAMURAL RES SUPPORT PROGRAM SCI APPLICAT INT CORP FREDERICK CANC RES & DEV CTR FREDERICK, MD 21702 USA NCI CYTOKINE MOL MECHANISMS SECT LAB MOL IMMUNOREGULAT FREDERICK CANC RES & DEV CTR FREDERICK, MD 21702 USA
    1. Year: 1997
  2. Journal: Febs Letters
    1. 408
    2. 3
    3. Pages: 327-330
  4. Type of Article: Article
  1. Abstract:

    Microinjection of expression plasmids encoding either JAK2 or hyperactive (N Delta 661)rJAK2 into serum-starved NIH 3T3 cells resulted in 20-30-fold induction of DNA synthesis, Control microinjections of buffer or parental pcDNA3 vector resulted in only 3-5-fold induction of DNA synthesis. Induction of DNA synthesis was blocked when plasmid encoding JAK2 was microinjected in the presence of the JAK2-selective inhibitor AG-490, whereas AG-490 did not block DNA synthesis induced by microinjected plasmid encoding (N Delta 661)rJAK2. The ability of JAK2 to initiate the G(o)/S cell cycle transition is comparable to that of other proto-oncogenes, and supports a mechanistic role for overexpressed Janus kinases in carcinogenesis. (C) 1997 Federation of European Biochemical Societies. [References: 24]

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