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Highly Efficient Autologous HIV-1 Isolation by Coculturing Macrophage With Enriched CD4+ T Cells From HIV-1 Patients

  1. Author:
    Xufré, Cristina
    González, Tanía
    Leal, Lorna
    Trubey, Charles M
    Lifson,Jeffrey
    Gatell, José María
    Alcamí, José
    Climent, Núria
    García, Felipe
    Sánchez-Palomino, Sonsoles

  2. Author Address

    AIDS Research Group, Institut d 39;Investigacions Biom 232;diques August Pi I Sunyer (IDIBAPS), Hospital Clinic, University of Barcelona, Barcelona, Spain., Centro de Investigaci 243;n Biom 233;dica en Red (CIBER) of Infectious Diseases, Centro de Investigaci 243;n Biom 233;dica en Red de Enfermedades Infecciosas, (CIBERINFEC), Instituto de Salud Carlos III, Madrid, Spain., Infectious Diseases Service, Hospital Clinic, Institut d 39;Investigacions Biom 232;diques August Pi I Sunyer (IDIBAPS), University of Barcelona, Barcelona, Spain., AIDS and Cancer Virus Program Inc., Frederick National Laboratory, Frederick, MD, United States., AIDS Immunopathology Unit, National Center of Microbiology, Instituto de Salud Carlos III, Madrid, Spain.,
    1. Year: 2022
    2. Date: Apr
    3. Epub Date: 2022 04 07
  2. Journal: Frontiers in Virology
    1. 2
  4. Type of Article: Article
  1. Abstract:

    We described a novel HIV autologous isolation method based in coculturing macrophages and CD4+T-cell-enriched fractions from peripheral blood collected from antiretroviral-treated (ART) HIV patients. This method allows the isolation of high viral titers of autologous viruses, over 1010HIV RNA copies/ml, and reduces the time required to produce necessary amounts for virus for use as antigens presented by monocyte-derived myeloid cells in HIV therapeutic vaccine approaches. By applying these high titer and autologous virus produced in the patient-derived cells, we intended to elicit a boost of the immunological system response in HIV therapeutic vaccines in clinical trials.

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External Sources

  1. View Full Text
  2. DOI: 10.3389/fviro.2022.869431
  3. PMID: 35967461
  4. PMCID: PMC9364968

Library Notes

  1. Fiscal Year: FY2021-2022
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