Skip NavigationSkip to Content
Return Return to Search Results

Varying effects of temperature, Ca2+ and cytochalasin on fusion activity mediated by human immunodeficiency virus type 1 and type 2 glycoproteins

  1. Author:
    Jernigan, K. M.
    Blumenthal, R.
    Puri, A.

  2. Author Address

    Puri A NCI, Frederick Canc Res & Dev Ctr, Div Basic Sci, LECB,NIH POB B,Bldg 469,Rm 211,Miller Dr Frederick, MD 21702 USA NCI, Frederick Canc Res & Dev Ctr, Div Basic Sci, LECB,NIH Frederick, MD 21702 USA
    1. Year: 2000
  2. Journal: Febs Letters
    1. 474
    2. 2-3
    3. Pages: 246-251
  4. Type of Article: Article
  1. Abstract:

    We examined fusion mediated by the human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) envelope glycoproteins under various experimental conditions. Incubation of HeLa cells expressing HIV-2(ROD) and HIV-2(SBL,ISY) envelope glycoproteins with HeLa-CD4 target cells resulted in fusion at temperatures greater than or equal to 25 degrees C whereas fusion with cells expressing HIV-1(Lai) occurred only at greater than or equal to 31 degrees C. HIV-2 envelope glycoprotein-mediated fusion proceeded in the absence of Ca2+ in the culture medium, whereas HIV-1 fusion required Ca2+ ions for fusion, In contrast to HIV-2 envelope glycoprotein fusion, incubations in the presence of the 0.5 mu M cytochalasin B completely inhibited HIV-1 envelope glycoprotein-mediated fusion. Our results suggest that in contrast to HIV-2, HIV-1 fusion is dependent on dynamic processes in the target membrane. (C) 2000 Federation of European Biochemical Societies. [References: 27]

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel