Skip NavigationSkip to Content
Return Return to Search Results

Expression of a Murine Leukemia Virus Gag-Escherichia Coli Rnase Hi Fusion Polyprotein Significantly Inhibits Virus Spread

  1. Author:
    Vanbrocklin, M.
    Ferris, A. L.
    Hughes, S. H.
    Federspiel, M. J.

  2. Author Address

    Federspiel MJ MAYO CLIN & MAYO FDN PROGRAM MOL MED 200 1ST ST SW ROCHESTER, MN 55905 USA MAYO CLIN & MAYO FDN PROGRAM MOL MED ROCHESTER, MN 55905 USA NCI ADV BIOSCI LABS BASIC RES PROGRAM FREDERICK CANC RES & DEV CTR BETHESDA, MD 21702 USA
    1. Year: 1997
  2. Journal: Journal of Virology
    1. 71
    2. 4
    3. Pages: 3312-3318
  4. Type of Article: Article
  1. Abstract:

    The antiviral strategy of capsid-targeted viral inactivation (CTVI) was designed to disable newly produced virions by fusing a Gag or Gag-Pol polyprotein to a degradative enzyme (e.g., a nuclease or protease) that would cause the degradative enzyme to be inserted into virions during assembly. Several new experimental approaches have been developed that increase the antiviral effect of the CTVI strategy on retroviral replication in vitro. A Moloney murine leukemia virus (Mo-MLV) Gag-Escherichia coli RNase HI fusion has a strong antiviral effect when used prophylacitcally, inhibiting the spread of Mo-MLV and reducing virus titers 1,500- to 2,500-fold, A significant (similar to 100-fold) overall improvement of the CTVI prophylactic antiviral effect was produced by a modification in the culture conditions which presumably increases the efficiency of delivery and expression of the Mo-MLV Gag fusion polyproteins. The therapeutic effect of Mo-MLV Gag-RNase HI polyproteins is to reduce the production of infectious Mo-MLV up to 18-fold, An Mo-MLV Gag-degradative enzyme fusion junction was designed that can be clear ed by the Mo-MLV pretense to release the degradative enzyme. [References: 34]

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel