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In Vitro Reconstitution of the Drosophila melanogaster CCR4-NOT Complex to Assay Deadenylation

  1. Author:
    Pekovic,Filip
    Wahle, Elmar

  2. Author Address

    Institute of Biochemistry and Biotechnology and Charles Tanford Protein Center, Martin Luther University Halle-Wittenberg, Halle, Germany. filip.pekovic@nih.gov., RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA. filip.pekovic@nih.gov., Institute of Biochemistry and Biotechnology and Charles Tanford Protein Center, Martin Luther University Halle-Wittenberg, Halle, Germany. ewahle@biochemtech.uni-halle.de.,
    1. Year: 2024
  2. Journal: Methods in Molecular Biology (Clifton, N.J.)
    1. 2723
    2. Pages: 19-45
  4. Type of Article: Article
  1. Abstract:

    The CCR4-NOT complex is a multi-subunit poly(A)-specific 3' exoribonuclease that catalyzes the deadenylation of mRNA. In this chapter, we describe procedures to express and purify recombinant Drosophila melanogaster CCR4-NOT. Furthermore, we provide protocols for preparing radioactively labeled RNA substrates and conducting in vitro deadenylation assays. © 2024. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.

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External Sources

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  2. DOI: 10.1007/978-1-0716-3481-3_2
  3. PMID: 37824062

Library Notes

  1. Fiscal Year: FY2023-2024
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