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Reconstitution of Human CCR4-NOT Complex from Purified Proteins and an Assay of Its Deadenylation Activity

  1. Author:
    Levdansky,Yevgen
    Valkov,Eugene

  2. Author Address

    RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA., RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA. eugene.valkov@nih.gov.,
    1. Year: 2024
  2. Journal: Methods in Molecular Biology (Clifton, N.J.)
    1. 2723
    2. Pages: 1-17
  4. Type of Article: Article
  1. Abstract:

    We describe protocols to produce and reconstitute an active human CCR4-NOT complex. Individual recombinant subunits are expressed in E. coli or baculovirus-infected insect cells, purified using column chromatography, and reconstituted into a stable complex containing all eight core subunits. In addition, we describe the biochemical assay of deadenylation using the reconstituted complex. © 2024. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.

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  2. DOI: 10.1007/978-1-0716-3481-3_1
  3. PMID: 37824061

Library Notes

  1. Fiscal Year: FY2023-2024
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