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Gene profiling approach in the analysis of lymphotoxin and TNF deficiencies

  1. Author:
    Shakhov, A. N.
    Lyakhov, I. G.
    Tumanov, A. V.
    Rubtsov, A. V.
    Drutskaya, L. N.
    Marino, M. W.
    Nedospasov, S. A.

  2. Author Address

    Shakhov AN NCI, Frederick Canc Res & Dev Ctr, IRSP, SAIC Frederick Bldg 560,Rm 31-33 Frederick, MD 21702 USA NCI, Frederick Canc Res & Dev Ctr, IRSP, SAIC Frederick Frederick, MD 21702 USA NCI, Frederick Canc Res & Dev Ctr, Div Basic Sci, Lab Mol Immunoregulat Frederick, MD 21702 USA Russian Acad Sci, VA Engelhardt Mol Biol Inst, Lab Mol Immunol Moscow Russia Moscow State Univ, Belozersky Inst Physicochem Biol Moscow Russia Mem Sloan Kettering Canc Ctr, Ludwig Inst Canc Res, New York Branch New York, NY 10021 USA
    1. Year: 2000
  2. Journal: Journal of Leukocyte Biology
    1. 68
    2. 1
    3. Pages: 151-157
  4. Type of Article: Article
  1. Abstract:

    Mice with combined lymphotoxin-alpha (LT alpha) and tumor necrosis factor (TNF) deficiencies show defects hi the structure of peripheral lymphoid organs such as spleen, lymph nodes, and gut-associated lymphoid tissues. To identify genes associated with this defective phenotype in spleen. we applied a gene profiling approach, including subtractive cloning and gene array hybridizations, to mice with combined TNF/LT deficiency. The differentially expressed genes identified by these techniques was then evaluated by Northern blot analysis for splenic expression ill knockout mice with single LT alpha or single TNF deficiency. Most of the genes detected in this analysis are directly or indirectly associated with disrupted LT and not TNF signaling. [References: 24]

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