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A novel putative transcription factor protein MYT2 that preferentially binds supercoiled DNA and induces DNA synthesis in quiescent cells

  1. Author:
    Shao, W.
    Lee, A. Y.
    Gulnik, S.
    Gustchina, E.
    Liu, Y. L.
    Kung, H. F.
    Erickson, J. W.

  2. Author Address

    Shao W Celera Genom 45 W Gude Dr Rockville, MD 20850 USA NCI, Struct Biochem Program, SAIC Frederick, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA NCI, Lab Biochem Physiol Frederick, MD 21702 USA Univ Hong Kong, Inst Mol Biol Hong Kong Hong Kong Peoples R China
    1. Year: 2000
  2. Journal: Febs Letters
    1. 473
    2. 3
    3. Pages: 363-369
  4. Type of Article: Article
  1. Abstract:

    Myelin transcription factor 2 (MYT2), a putative transcription factor found in the human central nervous system, was cloned from an expression cDNA library from human T-cells, MYT2 shares weak similarity to bacterial type I topoisomerases and shares 63% sequence identity to a replicase from Leuconostoc mesenteroides. MYT2 preferentially binds supercoiled DNA (scDNA), Incubation of MYT2 and scDNA at or above equal molar ratios generated topoisomer-like patterns that were abolished by deproteination. Thus, MYT2 appears to relax scDNA via a non-enzymatic mechanism. The banding pattern of MYT2-scDNA complexes was shown to be quantisized, saturable and sequence-independent. Microinjection of MYT2 mRNA induced G(0) growth-arrested NIH 3T3 cells to enter the S phase of the cell cycle, (C) 2000 Federation of European Biochemical Societies. [References: 34]

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